Constitutive integrin endocytosis and recycling control cell movement and morphology. In contrast, the role of newly synthesized integrins delivered via the biosynthetic pathway has been largely overlooked. We used the retention using selective hooks system to monitor the localization of…
Calponin isoforms define the cell-type-specific organization and dynamics of actomyosin bundles by Shrikant B Kokate et al.
Contractile actomyosin bundles, stress fibers, are important for cell migration, adhesion, morphogenesis, and mechanosensing. Calponin (CNN) family proteins are abundant stress fiber components, but their cellular functions and isoform-specific roles remain poorly understood. By depleting the three CNN isoforms (calponin-1…
Hepatic zonation determines tumorigenic potential of mutant β-catenin by Alexander Raven et al.
Oncogenic mutations in phenotypically normal tissue are common across adult organs^(1,2). This suggests that multiple events need to converge to drive tumorigenesis and that many processes such as tissue differentiation may protect against carcinogenesis. WNT-β-catenin signalling maintains zonal differentiation during…
COVID-19 vaccine type controls stromal reprogramming in draining lymph nodes by Ruth Fair-Mäkelä et al.
Lymph node (LN) stromal cells are critical regulators of immune reactions, yet their responses to different SARS-CoV-2 vaccines remain unexplored. Here, we immunized mice with clinically approved gene- and protein-based COVID-19 vaccines targeting viral spike (S) protein and analyzed the…
RAB5A Promotes Active Fluid Wetting by Reprogramming Breast Cancer Spheroid Mechanics by Grégoire Lemahieu et al.
Unjamming transitions from a solid-like to a fluid-like state are a gateway to breast epithelial cancer invasion. However, the mechanical interplay between phase transitions and dimension transitions, in particular wetting, remains elusive, despite being critical for understanding the onset of…
EPLINα controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration by Niklas Z Jäntti et al.
Epithelial protein lost in neoplasm (EPLIN), an actin-binding protein, has been described as both a tumor promoter and tumor suppressor in different cancers. The roles of EPLIN isoforms (α/β) remain largely unknown and could explain these opposing views. We observed…
Integrin β1 activity controls colony morphology during human pluripotent stem cell state transitions by Maria E Taskinen et al.
Integrin β1-mediated adhesion is dispensable in early mouse embryogenesis (pre-implantation) but indispensable post-implantation, suggesting distinct roles for β1-integrin-mediated adhesions in the naive (pre-implantation) versus primed (post-implantation) pluripotent stem cells (PSCs). We investigated the role of integrin β1 in regulating naive-like…
Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells by James R W Conway et al.
Integrins provide an essential bridge between cancer cells and the extracellular matrix, playing a central role in every stage of disease progression. Despite the recognized importance of integrin phosphorylation in several biological processes, the regulatory mechanisms and their relevance remained…
mTORC1 shutdown unleashes TFEB to drive triple-negative breast cancer invasion by Michalis Gounis et al.
The PI3K/AKT/mTOR pathway is considered a key therapeutic target in triple-negative breast cancer (TNBC). In this issue of Developmental Cell, Remy et al. challenge this idea by demonstrating that mTORC1 inhibition activates TFEB, promoting MT1-MMP exocytosis, ECM degradation, and increased…