We also focus on identifying integrin-specific regulators that impinge on integrin trafficking pathways to provide a means to selectively target integrins. We have adopted several techniques to study integrin trafficking including the retention using selective hooks (RUSH) system, which can be used to study synchronised receptor recycling under different conditions (e.g. on different extracellular matrix ligands; drug stimulation; loss or gain of function experiments). In addition, we have performed both siRNA screens and comprehensive mass spectrometric analyses of integrin trafficking regulators and our most recent work has identified key roles for actin-binding protein swiprosin-1 in directing integrin endocytosis to the CG-pathway thus promoting integrin endocytosis and cell migration. Accordingly, high levels of swiprosin-1 correlates with a significant increase in breast cancer metastasis in large cohort of triple-negative breast cancer.
Related publication from the lab
- Moreno-Layseca et al., Nature Cell Biology (2021)
- Sahgal P et al., Journal of Cell Science (2019)
- Moreno-Layseca et al., Nature Cell Biology (review) (2019)